The cleanPCR kit is an efficient PCR1) and Next Gen library prep clean-up system based on paramagnetic beads technology, providing an efficient purification of PCR amplicons. With its simple, three-step protocol, CleanPCR removes salts, primers, primer-dimers and dNTPs, while DNA fragments are selectively bound to the magnetic particles; highly purified DNA is eluted with low salt elution buffer or water and can be used directly for downstream applications. The protocol can be adapted to your current liquid handling workstation (e.g. Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol as well as being used manually.
There are several features that come with the cleanPCR kit. The features are: high recovery of amplicons greater than 100 bp, efficiently removes unincorporated dNTPs, primers, primer dimers and other contaminants, stable and high recovery of PCR products post-cleanup and no centrifugation or filtration.
– PCR 1)
– Mutation detection and Genotyping
– Sequencing (Sanger and Next Generation)
– Fragment Analysis
– Restriction enzyme clean up
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